Synthesis of florisil materials modified with aliphatic or aromatic groups and the application for pipette-tip solid-phase extraction of rutin in orange peel.

After being coupled, florisil was modified with four kinds of aliphatic or aromatic groups and were applied to the determination of rutin in orange peel sample by a pipette-tip solid-phase extraction method. The materials were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis and specific surface area by Brunauer Emmett Teller N2 adsorption desorption analysis. Florisil and five modified materials were tested by static adsorption, and the result showed that the material with 4-bromoanisole had the best adsorption amount for rutin. Under optimum conditions: 4 mg of the adsorbent, 1 mL of MeOH as washing solvent, and 1 mL of MeOH/acetic acid (85:15, v/v) as elution solvent were used in the pipette-tip solid-phase extraction procedure. The calibration graphs of rutin in MeOH were linear over 1-200 μg/mL, and the limits of detection and quantification were 0.13 and 0.42 μg/mL. The method recoveries of rutin were in the range of 83.66-98.40% with associated intra- and interday relative standard deviations in the range of 1.31-3.50% and 1.04-2.03%, respectively. The material with 4-bromoaniline showed satisfactory results (recovery: 98.40%). It was potential for the rapid purification of rutin in orange peel sample combined with pipette-tip solid-phase extraction.