- A rapid and sensitive assay for histone acetyl-transferase activity.
A rapid and sensitive assay for histone acetyl-transferase activity.
Nucleic acids research (1998-08-01)
S Ait-Si-Ali, S Ramirez, P Robin, D Trouche, A Harel-Bellan
PMID9685509
ABSTRACT
Histone acetyl-transferases (HATs) seem to be key elements in the regulation of transcription. We have designed an enzymatic assay to quantify HAT enzymatic activity. In this assay, the substrate is a peptide corresponding to the 24 first amino acids of histone H4 which is coupled to biotin. After acetylation using [14C]acetyl-CoA, the peptide is purified on streptavidin beads and the associated radioactivity is measured. This assay is sensitive, rapid and convenient.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Histone H4 Peptide, biotin conjugate, residues 2-24, Histone H4 Peptide, biotin conjugate, residues 2-24 primarily used in Immunoprecipitation and Affinity Precipitation Assays.