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A fluorescent probe for imaging p53-MDM2 protein-protein interaction.

Chemical biology & drug design (2014-09-18)
Zhenzhen Liu, Zhenyuan Miao, Jin Li, Kun Fang, Chunlin Zhuang, Lupei Du, Chunquan Sheng, Minyong Li
ABSTRACT

In this article, we describe a no-wash small-molecule fluorescent probe for detecting and imaging p53-MDM2 protein-protein interaction based on an environment-sensitive fluorescent turn-on mechanism. After extensive biological examination, this probe L1 exhibited practical activity and selectivity in vitro and in cellulo.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-MDM2 Antibody, clone 3G9, clone 3G9, from mouse