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  • Discovery of 3,5-bis(trifluoromethyl)benzyl L-arylglycinamide based potent CCR2 antagonists.

Discovery of 3,5-bis(trifluoromethyl)benzyl L-arylglycinamide based potent CCR2 antagonists.

Bioorganic & medicinal chemistry letters (2006-05-16)
Lihu Yang, Changyou Zhou, Liangqin Guo, Gregori Morriello, Gabor Butora, Alexander Pasternak, William H Parsons, Sander G Mills, Malcolm MacCoss, Pasquale P Vicario, Hans Zweerink, Julia M Ayala, Shefali Goyal, William A Hanlon, Margaret A Cascieri, Marty S Springer
ABSTRACT

Systematic modification of a screening lead yielded a class of potent glycinamide based CCR2 antagonists. The best compound (55, (2S)-N-[3,5-bis(trifluoromethyl)benzyl]-2-{[2-(1-piperidinyl)ethyl]amino}-2-(3-thienyl)acetamide) displayed good binding affinity (IC50=30 and 39 nM) toward human monocytes and CHO cell expressing human CCR2b, respectively. Functionally, it blocked MCP-1 (CCL2)-induced calcium mobilization (IC50=50 nM) and chemotaxis mediated through the CCR2 receptor (9.6 nM). It is selective against other chemokine receptors tested.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Glycinamide hydrochloride, 98%
Sigma-Aldrich
3,5-Bis(trifluoromethyl)benzylamine, technical grade, 80%
Sigma-Aldrich
Glycinamide hydrochloride, ≥99.0% (AT)