- Efficient biotinylation of methacryloyl-functionalized nonadherent cells for formation of cell microarrays.
Efficient biotinylation of methacryloyl-functionalized nonadherent cells for formation of cell microarrays.
Chemical communications (Cambridge, England) (2011-08-20)
Yasuhiko Iwasaki, Tatsuya Ota
PMID21853201
ABSTRACT
In order to obtain cell microarrays formed with human promyelocytic leukemia cells (HL-60), biotinylation of methacryloyl-functionalized HL-60 cells was performed via a thiol-ene reaction with thiol-terminated 4-arm poly(ethylene glycol) (PEG(4)10K-SH). Biotinylated HL-60 cells were selectively adhered onto an avidin-patterned surface with high viability.
MATERIALS