- Methods for the determination of plasma or tissue glutathione levels.
Methods for the determination of plasma or tissue glutathione levels.
Methods in molecular biology (Clifton, N.J.) (2012-06-07)
Trent E Tipple, Lynette K Rogers
PMID22669674
ABSTRACT
We present two different methods for determining levels of glutathione in complex biological samples and plasma. The DTNB/GR enzyme recycling method is sensitive and requires no specialized equipment. The HPLC method is particularly useful for situations in which sample amounts are limited. Detailed instructions for performing each method as well as the advantages and disadvantages of each are discussed in this chapter.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
5,5′-Dithiobis(2-nitrobenzoic acid), ≥98%, BioReagent, suitable for determination of sulfhydryl groups
Sigma-Aldrich
β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate, ≥93% (HPLC)
Sigma-Aldrich
Dansyl chloride, BioReagent, suitable for amino acid labeling, powder and chunks, ≥99% (HPLC)