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Chemical dehydration for rapid paraffin embedding.

Biotechnic & histochemistry : official publication of the Biological Stain Commission (1994-09-01)
W Möller, G Möller
PMID7819424
ABSTRACT

We describe chemical dehydration with 2,2-dimethoxypropane (DMP) for rapid paraffin embedding using a mixture of DMP and mineral oil followed by mineral oil as clearing intermediates. This method is useful for classical histological techniques as well as for histochemistry and immunocytochemistry.

MATERIALS
Product Number
Brand
Product Description
73137
Supelco
2,2-Dimethoxypropane, analytical standard
D136808
Sigma-Aldrich
2,2-Dimethoxypropane, reagent grade, 98%