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  • Rational engineering of Candida antarctica lipase B for selective monoacylation of diols.

Rational engineering of Candida antarctica lipase B for selective monoacylation of diols.

Chemical communications (Cambridge, England) (2012-09-05)
Anders Hamberg, Steffen Maurer, Karl Hult
ABSTRACT

The enzyme Candida antarctica lipase B was subjected to site directed mutagenesis suggested by molecular modelling. The selectivity for the enzyme increased towards a range of diols over their corresponding monoesters as an effect of the mutations.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
1,4-Butanediol, ReagentPlus®, 99%
Sigma-Aldrich
1,4-Butanediol, ReagentPlus®, ≥99%