- The influence of bile salts and bile lipoprotein complex on pancreatic lipase hydrolysis of monomolecular films.
The influence of bile salts and bile lipoprotein complex on pancreatic lipase hydrolysis of monomolecular films.
Biochimica et biophysica acta (1980-04-18)
D Lairon, M Charbonnier-Augeire, G Nalbone, J Leonardi, J C Hauton, G Pieroni, F Ferrato, R Verger
PMID7378424
ABSTRACT
We report a new technique which allows us to follow the lipolysis of monomolecular films in the presence of bile salts by using a 'zero-order' trough (Verger, R. and de Haas, G.H. (1973) Chem. Phys. Lipids 10, 127). The effects of bile salts, the bile lipoprotein complex and colipase on pancreatic lipase hydrolysis of rac-1,2-didodecanoylglycerol films were studied at different surface pressures. Taking into account previous studies, lipase activity was interpreted as a function of its degree of binding to the bile lipoprotein complex.
MATERIALS