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  • Fast detection of alpha-fetoprotein-L3 using lens culinaris agglutinin immobilized gold nanoparticles.

Fast detection of alpha-fetoprotein-L3 using lens culinaris agglutinin immobilized gold nanoparticles.

Journal of nanoscience and nanotechnology (2014-04-18)
Ying Sun, Liubin Qin, Dejun Liu, Changhong Liu, Yongwei Sun, Yourong Duan
PMID24738354
ABSTRACT

Colloidal gold is extensively used for molecular sensing because that the surface plasmon resonance (SPR) bands are affected by changes in the dielectric properties in the close vicinity of these structures due to the binding of ligands to the corresponding receptor molecules immobilized onto the nanostructures through chemi- or physisorption. We describe a simple method for the detection of Alpha-Fetoprotein-L3 which is a new generation of tumor marker for hepatocellular carcinoma (HCC) based on the aggregation of Lens culinaris agglutinin (LCA) Immobilized Gold Nanoparticles. The LCA conjugated GNPs obtained were 15-20 nm in diameter. The visible color change of the gold nanoparticles from purple to blue on interaction with 100 ng/mL of AFP-L3 is the principle applied here for the sensing of AFP-L3 level. UV/Vis spectroscopy also allows assay monitoring by quantifying the red shift of the plasmon resonance wavelength. With this method, the protein AFP-L3 can be rapidly detected as demanded for clinical diagnosis.

MATERIALS
Product Number
Brand
Product Description
265837
Sigma-Aldrich
Gold, rod, diam. 3.0 mm, 99.99% trace metals basis
373168
Sigma-Aldrich
Gold, evaporation slug, diam. × L 0.6 cm × 0.6 cm, 99.99% trace metals basis
GF92960286
Gold, tube, 50mm, outside diameter 8mm, inside diameter 7.8mm, wall thickness 0.1mm, as drawn, 99.95%
GF38598980
Gold, microfoil, disks, 10mm, thinness 0.5μm, specific density 966μg/cm2, permanent mylar 3.5μm support, 99.99+%
GF98812097
Gold, rod, 6mm, diameter 6.0mm, as drawn, 99.95%
GF26034320
Gold, tube, 100mm, outside diameter 10.0mm, inside diameter 9.7mm, wall thickness 0.15mm, as drawn, 99.95%
GF03557336
Gold, rod, 10mm, diameter 6.0mm, as drawn, 99.95%
GF37996789
Gold, tube, 200mm, outside diameter 2.0mm, inside diameter 1.7mm, wall thickness 0.15mm, as drawn, 99.95%
GF50454132
Gold, insulated wire, 2m, conductor diameter 0.125mm, insulation thickness 0.014mm, polyester insulation, 99.99%
GF75346678
Gold, insulated wire, 0.5m, conductor diameter 0.075mm, insulation thickness 0.012mm, PTFE (polytetrafluoroethylene) insulation, 99.99%
GF06182940
Gold, tube, 50mm, outside diameter 1.0mm, inside diameter 0.5mm, wall thickness 0.25mm, as drawn, 99.95%
GF04839767
Gold, tube, 100mm, outside diameter 1.60mm, inside diameter 0.6mm, wall thickness 0.5mm, as drawn, 99.95%
GF33293729
Gold, insulated wire, 0.1m, conductor diameter 0.125mm, insulation thickness 0.016mm, PTFE (polytetrafluoroethylene) insulation, 99.99%
GF47078129
Gold, microleaf disks, 10mm, thinness 0.5μm, specific density 966μg/cm2, removable support, 99.99+%
GF43173930
Gold, rod, 25mm, diameter 3.0mm, as drawn, 99.95%
GF41857409
Gold, insulated wire, 0.5m, conductor diameter 0.025mm, insulation thickness 0.005mm, polyester insulation, 99.99%
GF59949264
Gold, tube, 200mm, outside diameter 1.0mm, inside diameter 0.5mm, wall thickness 0.25mm, as drawn, 99.95%
GF96742479
Gold, rod, 50mm, diameter 3.0mm, as drawn, 99.95%
GF54796304
Gold, insulated wire, 1m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyester insulation, 99.99%
GF91804271
Gold, insulated wire, 2m, conductor diameter 0.075mm, insulation thickness 0.012mm, PTFE (polytetrafluoroethylene) insulation, 99.99%
GF92307922
Gold, insulated wire, 5m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyester insulation, 99.99%
GF45838985
Gold, insulated wire, 0.5m, conductor diameter 0.125mm, insulation thickness 0.016mm, PTFE (polytetrafluoroethylene) insulation, 99.99%
GF04885937
Gold, tube, 100mm, outside diameter 1.0mm, inside diameter 0.5mm, wall thickness 0.25mm, as drawn, 99.95%
GF46990974
Gold, microfoil, disks, 10mm, thinness 0.05μm, specific density 101.3μg/cm2, permanent mylar 3.5μm support, 99.99+%
GF39029968
Gold, rod, 100mm, diameter 2.0mm, as drawn, 99.95%
GF23782350
Gold, microfoil, disks, 10mm, thinness 0.1μm, specific density 204.1μg/cm2, permanent mylar 3.5μm support, 99.99+%
GF18334436
Gold, insulated wire, 1m, conductor diameter 0.075mm, insulation thickness 0.012mm, PTFE (polytetrafluoroethylene) insulation, 99.99%
GF73794741
Gold, tube, 200mm, outside diameter 2.0mm, inside diameter 1.8mm, wall thickness 0.1mm, as drawn, 99.95%
GF05586969
Gold, tube, 100mm, outside diameter 3.0mm, inside diameter 2.8mm, wall thickness 0.10mm, as drawn, 99.95%
GF22371319
Gold, rod, 25mm, diameter 4.0mm, as drawn, 99.95%