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  • Determination of phosphate in serum and urine by a single step malachite-green method.

Determination of phosphate in serum and urine by a single step malachite-green method.

Clinica chimica acta; international journal of clinical chemistry (1975-02-22)
A Kallner
ABSTRACT

The highly sensitive malachite-green method for the determination of phosphate requires a protective colloid in order to avoid precipitation of the formed dye salt. A polyvinylalcohol proved to be suitable and permit the determination of phosphate in urine and in serum without prior precipitation of the proteins. The present method is suitable for a one-step procedure which allows the analysis of several hundred samples per man-hour and is applicable to microquantities of serum or urine.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Malachite Green oxalate salt, for microscopy, crystalline, S. No.: 754