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  • Skeletal muscle perilipin 3 and coatomer proteins are increased following exercise and are associated with fat oxidation.

Skeletal muscle perilipin 3 and coatomer proteins are increased following exercise and are associated with fat oxidation.

PloS one (2014-03-19)
Jeffrey D Covington, Jose E Galgani, Cedric Moro, Jamie M LaGrange, Zhengyu Zhang, Arild C Rustan, Eric Ravussin, Sudip Bajpeyi
ABSTRACT

Lipid droplet-associated proteins such as perilipin 3 (PLIN3) and coatomer GTPase proteins (GBF1, ARF1, Sec23a, and ARFRP1) are expressed in skeletal muscle but little is known so far as to their regulation of lipolysis. We aimed here to explore the effects of lipolytic stimulation in vitro in primary human myotubes as well as in vivo following an acute exercise bout. In vitro lipolytic stimulation by epinephrine (100 μM) or by a lipolytic cocktail (30 μM palmitate, 4 μM forskolin, and 0.5 μM ionomycin, PFI) resulted in increases in PLIN3 protein content. Coatomer GTPases such as GBF1, ARF1, Sec23a, and ARFRP1 also increased in response to lipolytic stimuli. Furthermore, a long duration endurance exercise bout (20 males; age 24.0 ± 4.5 y; BMI 23.6 ± 1.8 kg/m(2)) increased PLIN3 protein in human skeletal muscle (p = 0.03) in proportion to ex vivo palmitate oxidation (r = 0.45, p = 0.04) and whole body in vivo fat oxidation (r = 0.52, p = 0.03). Protein content of ARF1 was increased (p = 0.04) while mRNA expression was increased for several other coatomers (GBF1, ARF1, and Sec23a, all p<0.05). These data provide novel observational insight into the possible relationships between lipolysis and PLIN3 along with these coatomoer GTPase proteins in human skeletal muscle.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Myosin Antibody, slow muscle, clone NOQ7.5.4D, clone NOQ7.5.4D, Chemicon®, from mouse
Sigma-Aldrich
ANTI-PLIN3 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution