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  • Chemical reactions within DNA duplexes. Cyanogen bromide as an effective oligodeoxyribonucleotide coupling agent.

Chemical reactions within DNA duplexes. Cyanogen bromide as an effective oligodeoxyribonucleotide coupling agent.

FEBS letters (1988-05-09)
N I Sokolova, D T Ashirbekova, N G Dolinnaya, Z A Shabarova
ABSTRACT

Cyanogen bromide was found to condense oligodeoxyribonucleotides on a complementary template in aqueous solution. Optimum conditions for this vigorous and effective reaction were developed. CNBr proved to be useful for incorporation of phosphoramidate or pyrophosphate internucleotide bonds in DNA duplexes.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Cyanogen bromide, 99.995% trace metals basis
Sigma-Aldrich
Cyanogen bromide, reagent grade, 97%