- Single genome amplification and direct amplicon sequencing of
Single genome amplification and direct amplicon sequencing of
Protocol exchange (2010-09-28)
Weimin Liu, Yingying Li, Martine Peeters, Julian Rayner, Paul Sharp, George Shaw, Beatrice Hahn
PMID25221670
ABSTRACT
Conventional PCR followed by molecular cloning and sequencing of amplified products is commonly used to test clinical specimens for target sequences of interest, such as viral, bacterial or parasite nucleic acids. However, this approach has serious limitations when used to analyze mixtures of genetically divergent templates