- Development and Validation of Monoclonal Antibody-Based Antigen Capture ELISA for Detection of Group A Porcine Rotavirus.
Development and Validation of Monoclonal Antibody-Based Antigen Capture ELISA for Detection of Group A Porcine Rotavirus.
Viral immunology (2017-04-18)
Atta Muhammad Memon, Anjuman Ara Bhuyan, Fangzhou Chen, Xiaozhen Guo, Harish Menghwar, Yinxing Zhu, Xugang Ku, Shuhua Chen, Zhonghua Li, Qigai He
PMID28414586
ABSTRACT
Porcine rotavirus-A (PoRVA) is one of the common causes of mild to severe dehydrating diarrhea, leading to losses in weaning and postweaning piglets. A rapid, highly specific, and sensitive antigen-capture enzyme-linked immunosorbent assay (AC-ELISA) was developed for detection of PoRVA, by using VP6 (a highly conserved and antigenic protein of group-A rotavirus)-directed rabbit polyclonal antibodies (capture antibody) and murine monoclonal antibodies (detector antibody). The detection limit of AC-ELISA was found to be equal to that of conventional reverse transcription-polymerase chain reaction (RT-PCR; about 10
MATERIALS
Product Number
Brand
Product Description