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  • Synthesis of reduced-size gold nanostars and internalization in SH-SY5Y cells.

Synthesis of reduced-size gold nanostars and internalization in SH-SY5Y cells.

Journal of colloid and interface science (2017-07-13)
Giacomo Dacarro, Piersandro Pallavicini, Serena Maria Bertani, Giuseppe Chirico, Laura D'Alfonso, Andrea Falqui, Nicoletta Marchesi, Alessia Pascale, Laura Sironi, Angelo Taglietti, Efisio Zuddas
ABSTRACT

The synthesis of large pentatwinned five-branched gold nanostars (GNS) has been modified so to obtain overall dimensions shrunk to 60% and a lower branches aspect ratio, leading to a dramatic blue shift of their two near-infrared (NIR) localized surface plasmon resonances (LSPR) absorptions but still maintaining one LSPR in the biotransparent NIR range. The interactions of polyethylene glycol (PEG) coated large and shrunk GNS with SH-SY5Y cells revealed that the large ones (DCI - diameter of the circumference in which GNS can be inscribed=76nm) are internalized more efficiently than the shrunk ones (DCI=46nm), correlating with a decreased cells surviving fraction.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Triton X-100, laboratory grade
Sigma-Aldrich
7-Mercapto-4-methylcoumarin, ≥97.0% (GC)