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Non-radioactive LATS in vitro Kinase Assay.

Bio-protocol (2017-11-07)
Audrey W Hong, Kun-Liang Guan
PMID29104896
ABSTRACT

This protocol describes a method to directly measure LATS activity by an in vitro kinase assay using YAP as a substrate.

MATERIALS
Product Number
Brand
Product Description
I5502
Sigma-Aldrich
Isopropyl β-D-1-thiogalactopyranoside, ≥99% (TLC)
SAB4200237
Sigma-Aldrich
Anti-Glutathione-S-transferase (GST) antibody, Mouse monoclonal, clone 2H3-D10, purified from hybridoma cell culture