GC Analysis of PCBs in Whole Cow Milk (Spiked) on SLB®-5ms (20 m x 0.18 mm I.D., 0.18 μm) after QuEChERS Cleanup using Supel™ QuE Z-Sep, Fast GC Analysis
Materials
analytical column
SPE tube or plate
standard
CONDITIONS
sample preparation
SPE (Solid Phase Extraction)
sample/matrix
10 g whole cow′s milk
extraction tube
Supel™ QuE Non-buffered tube (4 g magnesium sulfate, 1 g sodium chloride) (55294-U)
clean-up tube
Supel™ QuE Z-Sep 150 mg, magnesium sulfate (150 mg, part no. 63135) (55299-U)
column
SLB-5ms, 20 m x 0.18 mm I.D., 0.18 μm (28564-U)
oven
75 °C (1 min), 12 °C/min to 340 °C (10 min)
inj. temp.
250 °C
detector
μ-ECD, 340 °C
carrier gas
hydrogen, 1.2 mL/min, constant
injection
1 μL splitless (0.75 min)
liner
4 mm I.D. FocusLiner™ with taper
Description
General description
Reference: Stenerson, K.; Ye, M.; Halpenny, M., New Analytical Tools for the Determination of Persistent Organic Pollutants (POPs) in Fatty Food and Beverage Matrices Using QuEChERS Extraction/Cleanup and Gas Chromatography (GC) Analysis. Pittcon 2013. Sigma-Aldrich/Supelco publication T413034
Analysis Note
This application demonstrates the use of dispersive SPE or "QuEChERS" for the extraction and cleanup of whole cow′s milk for GC-ECD analysis of polychlorinated biphenyl (PCBs). Z-Sep sorbent was used in the cleanup step and found to reduce background and yield recoveries >70% for all PCB congeners evaluated except no. 209, which was recovered at 68%.
Extraction solvent: acetone:hexane (1:1), 20 mL
Clean-up volume: 1 mL
Legal Information
FocusLiner is a trademark of SGE Analytical Science Pty Ltd.
SLB is a registered trademark of Merck KGaA, Darmstadt, Germany
Supel is a trademark of Sigma-Aldrich Co. LLC