HPLC Analysis of Carnitine Enantiomers on Astec® CHIROBIOTIC® TAG
CONDITIONS
column
CHIROBIOTIC TAG, 25 cm x 4.6 mm I.D., 5 μm particles (14024AST)
mobile phase
[A] 5 mM ammonium acetate, pH 6; [B] methanol; (25:75, A:B)
flow rate
0.6 mL/min
column temp.
35 °C
detector
ESI(+)-MS (m/z = 162)
injection
5 μL
sample
1 μg/mL in mobile phase
Description
Legal Information
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC