HPLC Analysis of mPEG and mPEG-Peptide on Zenix® and Zenix®-C SEC
CONDITIONS
column
Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 μm particles (Z777033)
column
Zenix-C SEC-300, 30 cm x 7.8 mm, I.D., 3 μm particles (Z777092)
mobile phase
150 mM sodium phosphate buffer, pH 7.0
flow rate
1.0 mL/min
column temp.
25 °C
detector
UV, 214 nm
injection
20 μL
sample
4kD peptide and 20kD mPEG-MAL(methoxy-PEG-Maleimide) (6 mg/mL)
Description
Legal Information
Zenix is a registered trademark of Sepax Technologies