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HPLC Analysis of Triacylglycerols in Soybean Oil on Discovery® HS C18 after Sample Prep using Discovery® DSC-Si (Propionitrile Mobile Phase, ELSD Detection)

HPLC Analysis of Triacylglycerols in Soybean Oil on Discovery® HS C18 after Sample Prep using Discovery® DSC-Si (Propionitrile Mobile Phase, ELSD Detection) application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Discovery® HS C18 HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

SPE tube or plate

Product No.
Description
Pricing

Discovery® DSC-Si SPE Tube

bed wt. 1 g, volume 6 mL, pk of 30

mobile phase component

Product No.
Description
Pricing

Propionitrile

99%

standard

Product No.
Description
Pricing

Glyceryl trioleate

≥99%

Soybean oil

analytical standard

CONDITIONS

condition

6 mL hexane

SPE tube/cartridge

Discovery DSC-Si, 1 gm/6 mL (52656-U)

sample addition

0.12 g oil in 0.5 mL hexane

elution

10 mL hexane-diethyl ether 87-13 (v/v)

eluate post-treatment

vaporation to dryness, reconstituted into 2 mL acetone

column

Discovery HS C18, 25 cm x 4.6 mm I.D., 5 μm particles (568523-U)

mobile phase

propionitrile

flow rate

1.5 mL/min

pressure

1117 psi (77 bar)

column temp.

30 °C

detector

ELSD

injection

5 μL

Description

Analysis Note

Soybean oil was cleaned using Silica SPE and analyzed as 5% sample in acetone (w/v).

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany