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Extraction Tube	SupelQue Non-buffered tube 2 (55295-U)
Extraction Process	Transfer the solution into 50 mL plastic tube (55248-U); add 10 mL acetonitrile; shake for 1 minute by hand; add contents of the Supel QuE Non-Buffered Extraction Tube 2 (55295-U); (shake for 5 minutes using QuEChERS shaker (55278-U); centrifuge for 5 minutes at 5000 rpm; collect top acetonitrile layer; no cleanup is required.)
Column	Ascentis Express Biphenyl, 10cm x 2.1mm, 2.7 µm particles (64065-U)
Column Temp.	35 °C
Mobile Phase	(A) 0.1% TFA in water; (B) 0.1% TFA in acetonitrile
Gradient	47% B to 50% B in 13 min.; 100% B in 0.1 min; held at 100% B for 3 min; 47% B in 0.1 min; held at 47% B for 2.5 min.
Flow Rate	0.7 mL/min
Pressure	5000 psi (345 bar)
Injection	5 µL
Detector	UV, 220 nm

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Analysis Note	Dissolve in heated water, 1 gummy candy in 20 mL
Categories	Analytical Chromatography, Neurotransmitters
Featured Industry	Pharmaceutical (small molecule)
Legal Information	Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
Suitability	Application for HPLC

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