11684795910
Roche
In Situ Cell Death Detection Kit, Fluorescein
sufficient for ≤50 tests, suitable for detection
Synonym(s):
Fluorescein-12-UTP tetralithium salt, Fluorescein-5(6)-carboxamidocaproyl-(5-[3-aminoallyl]uridine 5′-triphosphate)
About This Item
Recommended Products
usage
sufficient for ≤50 tests
Quality Level
manufacturer/tradename
Roche
technique(s)
flow cytometry: suitable
application(s)
detection
storage temp.
−20°C
Related Categories
General description
The hallmark of apoptosis is DNA degradation, which in early stages, is selective to the internucleosomal DNA linker regions. The DNA cleavage may yield double-stranded and single-stranded DNA breaks (nicks). Both types of breaks can be detected by labeling the free 3′-OH termini with modified nucleotides (e.g., biotin-dUTP, DIG-dUTP, fluorescein-dUTP) in an enzymatic reaction. The enzyme terminal deoxynucleotidyl transferase (TdT) catalyzes the template-independent polymerization of deoxyribonucleotides to the 3′-end of single- and double-stranded DNA. This method has also been termed TUNEL (TdT-mediated dUTP-X nick end labeling). Alternatively, free 3′-OH groups may be labeled using DNA polymerases by the template-dependent mechanism called nick translation. However, the TUNEL method is considered to be more sensitive and faster.
Specificity
Application
Examples are:
- Detection of individual apoptotic cells in frozen and formalin-fixed tissue sections in basic research
- Determination of sensitivity of malignant cells to drug-induced apoptosis in cancer research
- Typing of cells undergoing cell death in heterogeneous populations by double staining procedures
Features and Benefits
- Sensitive: The direct labeling procedure using fluorescein-dUTP reduces background labeling
- Fast: The use of fluorescein-dUTP allows analysis of the samples directly after the TUNEL reaction
- Convenient: No secondary detection system required
- Accurate: Identification of apoptosis at a molecular level (DNA-strand breaks) and identification of cells at the very early stages of apoptosis
Packaging
Preparation Note
Mix well to equilibrate components.
Other Notes
Kit Components Only
- Enzyme Solution (TdT)
- Label Solution (fluorescein-dUTP)
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 2 - Carc. 1B Inhalation
Storage Class
6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk_germany
WGK 3
flash_point_f
does not flash
flash_point_c
does not flash
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Articles
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Protocols
In Situ Cell Death Detection Kit, Fluorescein Protocol & Troubleshooting
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