Cultrex^® 3-D Culture Matrix^™ Reduced Growth Factor Basement Membrane Extract, (2 x 5ml)

3D Culture is a cell culture method that provides cells with the necessary structure and signaling cues to direct reconstruction of the tissue architecture, and these methods provide physiologically predictive in vitro models for evaluating development and disease. Normal cells assemble into organiods that structurally resemble their emanating tissues, exhibit a polarized morphology, undergo cell cycle regulation, and produce tissue-specific proteins. While cancer cells assemble into tumor-like structures, lacking an organized architecture or cell cycle regulation and exhibiting tumor-specific markers depending on the extend of malignancy.

Solution in Dulbecco′s Modified Eagle′s medium without phenol red, with 10 ug/ml gentamicin sulfate.

Functional Assay:
Tube formation assay - BME promotes formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells.
3D Culture - Basement Membrane Extract promotes differentiation of a human epithelial cell line derived from mammary gland (MCF-10A) or human prostate (PC-3) into acinar structures.

Sterility Testing:
PathClear^® - Negative by PCR test for mycoplasma; 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses. No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP sterility testing guidelines. Endotoxin concentration = 8 EU/ml by LAL assay.

Storage Conditions and product stability:
Product is stable for a minimum of 3 months from date of shipment when stored at –20°C in a manual defrost freezer. For optimal stability, store at –80°C. Avoid freeze-thaw cycles.

3-D Culture Matrix is a trademark of Trevigen, Inc.

Cultrex is a registered trademark of Trevigen, Inc.

PathClear is a registered trademark of Trevigen, Inc.