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M5519

Sigma-Aldrich

Murashige and Skoog Basal Medium

powder, suitable for plant cell culture

Synonym(s):

MS0 Medium, MSO Medium, MS Basal Medium

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About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.72

form

powder

Quality Level

technique(s)

cell culture | plant: suitable

application(s)

agriculture

storage temp.

2-8°C

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General description

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).

Application

Murashige and Skoog medium has been used in plant tissue culture medium for plant transformation study.

Formula variant

With the macro- and micronutrients, and vitamins as described by Murashige and Skoog (1962).

Media Formulation

Quantity

Formulated to contain 4.4 grams of powder per liter of medium.

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog medium (M5519) contains the micronutrients and vitamins of the original classic formulation. It can be supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

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Warning

Hazard Classifications

Eye Irrit. 2 - Ox. Sol. 3

Storage Class

5.1B - Oxidizing hazardous materials

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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