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MAK002

Sigma-Aldrich

L-Amino Acid Quantitation Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):

L-Amino Acid Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.25

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric
fluorometric

storage temp.

−20°C

General description

The new L-Amino Acid Assay Kit, MAK490, is now available!

L-amino acids are essential molecules defined by the nature of their side-chain. There are 20 amino acids that are commonly used by most organisms for the production of proteins and other biologically relevant molecules. While amino acids can occur in either the D- or L-enantimer, only the L-form is used by cells.

Suitability

Suitable for quantitating L-amino acids in body fluids or purified samples.

Principle

In this assay, L-Amino Acid concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the L-Amino Acids present. Glycine is not detectable by this assay.

replaced by

Product No.
Description
Pricing

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Hazard Classifications

Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral - Aquatic Chronic 3 - Resp. Sens. 1 - Skin Sens. 1

Storage Class

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk_germany

WGK 3

flash_point_f

188.6 °F

flash_point_c

87 °C

Certificates of Analysis (COA)

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Jinrang Kim et al.
Endocrinology, 160(5), 979-988 (2019-04-03)
Plasma amino acids and their transporters constitute an important part of the feedback loop between the liver and pancreatic α-cell function, and glucagon regulates hepatic amino acid turnover. Disruption of hepatic glucagon receptor action activates the loop and results in
Alberts B, et al.
Molecular Biology in Plant Pathogenesis and Disease Management (2002)
Analysis of endogenous D-amino acid-containing peptides in metazoa.
Bai L, et al.
Bioanalytical reviews., 1(1), 7-24 (2009)
Alberts B, et al.
Molecular biology of the Cell. 4th Edition. (2002)
Hai-Bin Ruan et al.
Genes & development, 31(16), 1655-1665 (2017-09-15)
Starvation induces liver autophagy, which is thought to provide nutrients for use by other organs and thereby maintain whole-body homeostasis. Here we demonstrate that O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT) is required for glucagon-stimulated liver autophagy and metabolic adaptation to starvation.
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