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S4438

Sigma-Aldrich

SYBR® Green JumpStart™ Taq ReadyMix™

for quantitative PCR, MgCI2 in buffer

Synonym(s):

Hot start PCR master mix, sybr green PCR master mix, sybr green qPCR

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About This Item

MDL number:
MFCD03458666
UNSPSC Code:
41106300
NACRES:
NA.55

Quality Level

200

form

liquid

usage

sufficient for 100 reactions
 sufficient for 20 reactions
 sufficient for 500 reactions

feature

dNTPs included
hotstart

concentration

1.25 units/reaction (50 μL reaction volume)

technique(s)

qPCR: suitable

input

purified DNA

suitability

suitable for (quantitative PCR)

application(s)

agriculture

compatibility

ABI 7000
 ABI 7300
 ABI 7500 Fast
 ABI 7500
 ABI 7700
 ABI 7900 HT Fast
 ABI 7900 HT
 ABI 7900
 ABI StepOne
 ABI StepOnePlus
 ABI ViiA 7
Bio-Rad CFX384
 Bio-Rad CFX96
 Bio-Rad MJ Chromo4
 Bio-Rad MJ Opticon 2
Bio-Rad MJ Opticon
 Bio-Rad MiniOpticon
 Bio-Rad MyiQ
 Bio-Rad iCycler iQ
 Bio-Rad iQ 5
 Cepheid SmartCycler
 Eppendorf® Mastercycler ep realplex
Eppendorf® Mastercycler ep realplex2 S
 Illumina Eco qPCR
 Qiagen Corbett Rotor-Gene 3000
 Qiagen Corbett Rotor-Gene 6000
 Qiagen Corbett Rotor-Gene Q
 Roche LightCycler 480
Strategene Mx3000P
 Strategene Mx3005P
 Strategene Mx4000
for use with ABI 5700

detection method

SYBR® Green

shipped in

wet ice

storage temp.

−20°C

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General description

SYBR® Green I is ideal for quantifying any DNA sequence. The dye binds to double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout cycling.
SYBR® Green JumpStart™ Taq ReadyMix™ combines the performance enhancements of JumpStart Taq antibody for hot start PCR with SYBR Green I and the convenience of an easy-to-use ReadyMix solution. This ready-to-use mixture of SYBR Green I, JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and reaction buffer is provided in a 2× concentrate for ease of use. Simply add 25 μL of the 2× mix to DNA template, primers and water. The JumpStart Taq antibody inactivates DNA polymerase at room temperature. When the temperature is raised above 70 °C in the first denaturation step of the cycling process, the complex dissociates and the polymerase becomes fully active. JumpStart Taq DNA polymerase prevents non-specific amplification resulting in more accurate CT values.

Application

SYBR® Green JumpStart™ Taq ReadyMix™ has been used:

  • in quantitative PCR (qPCR) to amplify a single area of conservation from all variants of myelin basic protein (mbp), SRY-Box 10 (sox10), and β-actin genes
  • in quantitative real-time PCR to detect the accumulation of PCR product at every cycle of amplification
  • in routine qPCR amplifications of DNA template and cDNA template
  • for amplification of RNA by quantitative real-time reverse transcription-PCR (qRT-PCR)

Features and Benefits

  • Delivers the benefits of antibody-inactivated hot-start PCR with SYBR Green detection in a ReadyMix ideal for high throughput applications; only primers and template are required
  • JumpStart™ Taq DNA polymerase prevents amplification of non-specific products, resulting in increased efficiency and higher target yield
  • SYBR® Green JumpStart™ Taq ReadyMix for SYBR based qPCR is formulated with MgCl2 or packaged with a separate vial for ease of optimization. ReadyMixes are compatible with tube- and plate-based instruments
  • This master mix allows consistency from one reaction to the next
  • Designed to minimize contamination
  • Reduced primer dimers
  • Reduced set-up time as compared to manual or wax HotStart® methods
  • Allows for room temperature set-up and contains a fluorescent dye, which is ideal for qPCR applications

Packaging

Default reaction volume is 50 μL

20RXN is packaged as 1 X 500 μL
100RXN is packaged as 1 X 2.5 mL
400RXN is packaged as 1 X 10 mL
500RXN is packaged as 1 X 12.5 mL

Principle

SYBR Green JumpStart Taq ReadyMix is recommended for single product real-time amplification experiments and can also be used for PCR optimization prior to manufacture of fluorescent-labeled probes. Fluorescent labeled probes are not recommended for use with SYBR Green I dye.

SYBR Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. SYBR Green I has an excitation and emission maxima of 494 nm and 521 nm, respectively. The instrument settings for ROX reference dye are satisfactory for the measurement of the Reference Dye for Quantitative PCR. Specificity of Sigma′s SYBR based QPCR detection is greatly enhanced by the incorporation of a hot-start mediated taq polymerase, JumpStart Taq.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785.. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Eppendorf is a registered trademark of Eppendorf AG
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

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pictograms

Exclamation markEnvironment
GHS07,GHS09

signalword

Warning

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Bikem Soygur et al.
Human reproduction (Oxford, England), 31(7), 1455-1461 (2016-05-14)
As Syncytin 1 (human endogenous retrovirus (HERV-W)) is crucial for human embryo placentation is it expressed during preimplantation embryo development? Syncytin 1 was expressed mainly in trophoblast cells of the blastocyst particularly in cells underlying the inner cell mass (ICM).
T Y C Pang et al.
Neuroscience, 141(2), 569-584 (2006-05-24)
Huntington's disease is a fatal neurodegenerative disorder caused by a mutation of the huntingtin gene and involves progressive motor abnormalities (including chorea), cognitive deficits (dementia) as well as psychiatric symptoms. We have previously demonstrated that environmental enrichment slows the onset
Differential effects of voluntary physical exercise on behavioral and brain-derived neurotrophic factor expression deficits in Huntington?s disease transgenic mice
Pang TYC, et al.
Neuroscience, 141(2), 569-584 (2006)
Temporal dynamics of myelination in the zebrafish spinal cord
Buckley C E, et al.
Glia, 58(7), 802-812 (2010)
Karim Sorefan et al.
Nature, 459(7246), 583-586 (2009-05-30)
Local hormone maxima are essential for the development of multicellular structures and organs. For example, steroid hormones accumulate in specific cell types of the animal fetus to induce sexual differentiation and concentration peaks of the plant hormone auxin direct organ
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