T6567
Trypsin from porcine pancreas
BioReagent, Proteomics Grade, Dimethylated
Synonym(s):
Porcine Trypsin, Trypsin for Mass Spectropetry
About This Item
Recommended Products
biological source
Porcine pancreas
Quality Level
product line
BioReagent
solubility
1 mM HCl: soluble 1 mg/mL, clear, colorless
shipped in
wet ice
storage temp.
2-8°C
Related Categories
General description
Application
- In-gel protein digestion and MALDI-TOF mass spectrometry analysis
- Electrospray Ionization Mass Spectrometry (ESI-MS) analysis
- Surface proteome profiling of L. plantarum
- Gel Filtration, Ultracentrifugation, and Rotary Shadowing Electron Microscopy
- Mass spectrometry
Biochem/physiol Actions
related product
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
wgk_germany
WGK 1
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Certificates of Analysis (COA)
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Articles
Evaluation of Recombinant, Chemically Treated Trypsin in Proteomics and Protein Characterization Assays
The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.
Protocols
This procedure is for products with a specification for Trypsin activity using Na-Benzoyl-L-arginine ethyl ester (BAEE) as a substrate. The procedure is a continuous spectrophotometric rate determination (A253, Light path = 1 cm).
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