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42085

Sigma-Aldrich

1-Fluoro-2,4-dinitrobenzene

purum p.a., ≥98.0% (GC)

Synonym(s):

2,4-Dinitro-1-fluorobenzene, DNFB, DNPF, FDNB, Sanger reagent

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About This Item

Linear Formula:
(O2N)2C6H3F
CAS Number:
70-34-8
Molecular Weight:
186.10
Beilstein/REAXYS Number:
398632
EC Number:
200-734-3
MDL number:
MFCD00007056
UNSPSC Code:
12352100
PubChem Substance ID:
57650098
NACRES:
NA.22

grade

purum p.a.

Quality Level

100

assay

≥98.0% (GC)

form

liquid

refractive index

n20/D 1.569 (lit.)

bp

178 °C/25 mmHg (lit.)

mp

25-27 °C (lit.)

density

1.482 g/mL at 25 °C (lit.)

cation traces

Ca: ≤50 mg/kg
Cd: ≤50 mg/kg
Co: ≤50 mg/kg
Cu: ≤50 mg/kg
Fe: ≤50 mg/kg
K: ≤100 mg/kg
Na: ≤100 mg/kg
Ni: ≤50 mg/kg
Pb: ≤50 mg/kg
Zn: ≤50 mg/kg

storage temp.

2-8°C

SMILES string

[O-][N+](=O)c1ccc(F)c(c1)[N+]([O-])=O

InChI

1S/C6H3FN2O4/c7-5-2-1-4(8(10)11)3-6(5)9(12)13/h1-3H

InChI key

LOTKRQAVGJMPNV-UHFFFAOYSA-N

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General description

1-Fluoro-2,4-dinitrobenzene(FDNB, Sanger′s reagent) is used to identify the amino acid sequence. Thischemical is used to label the free amino groups in proteins and peptides. Itcan also be used to protect the amine group, and for phenol deoxygenation. Additionally,it is a very helpful reagent for nucleophilic substitution reactions tointroduce a chromophore group to amines, phenols, and thiols.

Application

1-Fluoro-2,4-dinitrobenzene is used as a derivatization agent:
  • For aminoglycoside compounds such as amikacine, tobramycine, gentamycine, sisomycine, neamine, neomycine-B and neomycine-C.
1-Fluoro-2,4-dinitrobenzene can also be used:
  • To examine the kinetic analysis and analytical uses of reaction with thiols in the presence of several surfactants.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 - STOT SE 3

target_organs

Respiratory system

wgk_germany

WGK 3

flash_point_f

327.2 °F

flash_point_c

164 °C

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A spectrophotometric method for the determination of lisinopril (LN) in single and multicomponent tablets also containing hydrochlorothiazide (HCT), based on the derivatization reaction with 1-fluoro-2,4-dinitrobenzene (FDNB, Sanger reagent) is described. Aqueous solutions of LN (4.5-27.2 x 10(-5) M) react with
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