Skip to Content
MilliporeSigma
All Photos(1)

Documents

803650

Sigma-Aldrich

SBAP (succinimidyl 3-(bromoacetamido)propionate)

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C9H11N2O5Br
CAS Number:
57159-62-3
Molecular Weight:
307.10
MDL number:
MFCD01863457
UNSPSC Code:
12352200
PubChem Substance ID:
329768824
NACRES:
NA.22

assay

≥90%

Quality Level

100

form

powder

mol wt

307.1

reaction suitability

reagent type: cross-linking reagent

storage condition

desiccated

solubility

DMSO or DMF: soluble

functional group

NHS ester
bromo

shipped in

ambient

storage temp.

2-8°C

SMILES string

O=C(CCC1=O)N1OC(CCNC(CBr)=O)=O

InChI

1S/C9H11BrN2O5/c10-5-6(13)11-4-3-9(16)17-12-7(14)1-2-8(12)15/h1-5H2,(H,11,13)

InChI key

WGMMKWFUXPMTRW-UHFFFAOYSA-N

Related Categories

General description

SBAP is a sulfhydryl-reactive and amine-reactive heterobifunctional crosslinker. The reagent′s NHS ester reacts with primary amines at pH 7-9 to form stable amide bonds, and the bromacetyl reacts with sulfhydryl groups at pH >7.5 to form stable thioether bonds This reagent is useful for preparing cyclic peptides and peptide conjugates because the spacer maintains peptide-like character in the crosslinked species.

Features and Benefits

  • Reactive groups: NHS ester and bromoacetyl
  • Reactive toward: amino and sulfhydryl groups
  • NHS ester reacts with primary amines at pH 7-9 to form a stable amide bond
  • Bromacetyl group reacts with sulfhydryl groups at pH > 7.5 to form stable thioether bonds
  • Non-cleavable
  • Water-insoluble (dissolve first in DMF or DMSO)
  • Spacer maintains peptide-like character in the crosslinked species
  • Resulting crosslink is susceptible to acid hydrolysis
  • Useful for preparing cyclic peptides and peptide conjugates

Caution

This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

J K Inman et al.
Bioconjugate chemistry, 2(6), 458-463 (1991-11-01)
A new amino acid derivative, N alpha-(tert-butoxycarbonyl)-N epsilon-[N-(bromoacetyl)-beta-alanyl]-L-lysine (BBAL), has been synthesized as a reagent to be used in solid-phase peptide synthesis for introducing a side-chain bromoacetyl group at any desired position in a peptide sequence. The bromoacetyl group subsequently

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service