809705
5-DBCO-PEG4-dUTP, 10mM Aqueous Solution
Synonym(s):
5-DBCO-PEG4-dUTP
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About This Item
Empirical Formula (Hill Notation):
C42H54N5O21P3
Molecular Weight:
1057.82
UNSPSC Code:
12352200
PubChem Substance ID:
NACRES:
NA.22
Recommended Products
assay
>95% (HPLC)
Quality Level
form
solution
reaction suitability
reaction type: click chemistry
solubility
Tris buffer: 100 mM (pH 7.5)
shipped in
wet ice
storage temp.
−20°C
SMILES string
O=C1NC(N([C@H]2C[C@@H]([C@@H](COP(O)(OP(O)(OP(O)(O)=O)=O)=O)O2)O)C=C1/C=C\CNC(CCOCCOCCOCCOCCNC(CCC(N3CC4=CC=CC=C4C#CC5=CC=CC=C53)=O)=O)=O)=O
Related Categories
Application
5-DBCO-PEG4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR with Taq polymerase. It is enzymatically incorporated into DNA/cDNA as a substitute for its natural counterpart dTTP. The resulting DBCO-functionalized DNA/cDNA can be labeled with azide-labeled molecules using copper-free click chemistry. This enables experiments such as introduction of a biotin group for purification tasks, introduction of a fluorescent group for detection, or crosslinking the DNA to azide-functionalized biomolecules such as proteins.
wgk_germany
WGK 2
Certificates of Analysis (COA)
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