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927899

Sigma-Aldrich

Gold nanoparticles

15 nm diameter, Ni-NTA functionalized, 1 ML, OD 50, in water

Synonym(s):

AuNP, GdNP, Gold NP

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About This Item

UNSPSC Code:
12141717
NACRES:
NA.22

Quality Level

100

description

Ni-NTA surface density: 1/nm2

concentration

in water

OD

50

core diam.

15 nm , coefficient of variance <12%

diameter

15 nm

λmax

520 nm

PDI

<0.200

functional group

Ni-NTA

storage temp.

2-8°C

InChI

1S/Au

InChI key

PCHJSUWPFVWCPO-UHFFFAOYSA-N

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General description

Ni-NTA functionalized gold nanoparticles are suitable for binding of proteins with a C-, or N-terminal 6X His tag. These gold nanoparticles allow for rapid generation of gold conjugates for subsequent use in downstream assays.
Ni-NTA functionalized gold nanoparticles are more than 95% spherical and have uniform size distribution (CV <12%).

Application

Ideal for development of gold conjugates for use in applications such as blotting, lateral flow assays, microscopy, and transmission electron microscopy (TEM).

Storage and Stability

When stored for a long period of time gold nanoparticles may sediment at the bottom of the vial, which is especially prominent for larger particle sizes. Prior to use, re-suspend the sedimented particles by thorough mixing until a homogenous solution is obtained.

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Photocurrent generation properties of Histag-photosystem II immobilized on nanostructured gold electrode.
Saini V, et al.
Thin Solid Films, 516(9), 2553-2557 (2008)
A generic approach to monofunctionalized protein-like gold nanoparticles based on immobilized metal ion affinity chromatography.
Raphaël Lévy et al.
Chembiochem : a European journal of chemical biology, 7(4), 592-594 (2006-02-14)

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