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QBD10374

Sigma-Aldrich

SPDP-dPEG®4-NHS ester

Synonym(s):

2,5-Dioxo-1-pyrrolidinyl 17-oxo-19-(2-pyridinyldithio)-4,7,10,13-tetraoxa-16-azanonadecanoate

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About This Item

Empirical Formula (Hill Notation):
C23H33N3O9S2
CAS Number:
1334177-95-5
Molecular Weight:
559.65
MDL number:
MFCD13185001
UNSPSC Code:
12352108
PubChem Substance ID:
329823921
NACRES:
NA.22

assay

>90%

form

solid or viscous liquid

reaction suitability

reactivity: thiol reactive
reagent type: cross-linking reagent
reactivity: amine reactive

functional group

NHS ester

polymer architecture

shape: linear
functionality: heterobifunctional

shipped in

ambient

storage temp.

−20°C

SMILES string

O=C(NCCOCCOCCOCCOCCC(ON1C(CCC1=O)=O)=O)CCSSC2=NC=CC=C2

Related Categories

Features and Benefits

SPDP-dPEG®4-NHS ester has an amine-reactive N-hydroxysuccinimide (NHS) ester at one end and a sulfhydryl-reactive 2-pyridyldithiol group at the opposite end. This has a single molecular weight PEG spacer arm, which confers greater water solubility to the crosslinker and linked proteins compared to crosslinkers having only hydrocarbon spacers. Protein aggregation and precipitation also are greatly reduced with SPDP-dPEG crosslinkers compared to crosslinkers that have only hydrocarbon spacers. dPEG spacer of specific length (23.1 Å) provides precise distance control between the SPDP moiety and the target molecule. Pyridyldithiol reagents produce disulfide-containing linkages that can be cleaved with reducing agents such as dithiothreitol (DTT). Crosslinking experiments with SPDP reagents are not limited to those involving proteins. Any of a variety of molecules with primary amines and sulfhydryl groups can be modified or crosslinked using SPDP reagents.

Legal Information

Products Protected under U.S. Patents # 7,888,536 B2
dPEG is a registered trademark of Quanta BioDesign

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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