03-0190-00
SMC® Human IL-18 High Sensitivity Immunoassay Kit
Synonym(s):
IGIF immunoassay kit, IL-18 Single Molecule Counting Kit, IL-18 ultra-sensitive immunoassay, Interleukin-18 immunoassay kit
About This Item
Recommended Products
species reactivity
mouse, feline, rat
manufacturer/tradename
SMC®
assay range
sensitivity: 0.09 pg/mL
(Lower Limit of Quantification)
inter-assay cv: 7%
intra-assay cv: 4%
(five normal plasma samples run in triplicate by 3 different operators on 3 different days.)
technique(s)
single molecule counting: suitable
storage temp.
2-8°C
Related Categories
General description
Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2) The Erenna and SMCxPRO® immunoassay systems detect analytes using Single Molecule Counting (SMC®) technology.
Specificity
Specificity to the following species recombinant proteins were tested with the following results:
Feline – 0.5% Mouse – 4% Rat – 0.3%
Cross Reactivity:
No cross-reactivity was seen with the following cytokines: IL-1a, IL-1ß, IL-1RA, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IL-17E, IL-7F, IL-22, IL-27.
Accuracy:
- Minimum of 5 serum samples spiked with average recovery of 99% with a range of 92-107%
- Minimum of 5 plasma samples spiked with average recovery of 95% with a range of 88-103%
Application
This new streamline kit has the following features including:
- Improved options for sample prep and incubation
- Lyophilization of the standard provides the kit at one storage condition
- Increased reagent volumes to accommodate automation
- Validated to run on both SMCxPRO® and Erenna
Research Category:
Inflammation & Immunology
Research Sub-Category:
- Inflammatory Disease,
- Immune Response
- Autoimmune disease
Packaging
Components
- Assay Buffer
- IL-18 Coated Beads
- Standard Diluent
- IL-18 Detection Antibody
- IL-18 Standard
- 10X Wash Buffer
- Buffer D
- Elution Buffer B
Legal Information
Disclaimer
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Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B
wgk_germany
WGK 3
Certificates of Analysis (COA)
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Related Content
See how you can enhance your immunology research through the power of high sensitivity immunoassays by harnessing Single Molecule Counting (SMC®) technology to precisely characterize interferons, interleukins, and immunoglobins.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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