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packaging
1 ea of 50 μg
Quality Level
manufacturer/tradename
Millipore
growth mode
N/A
technique(s)
RIP: suitable
cell culture | mammalian: suitable
flow cytometry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
shipped in
dry ice
storage temp.
-65 to -96°C
Application
Both mRNAs and non-coding RNAs interact with RNA-binding proteins and regulate critical cellular processes and their dysregulation contributes to multiple diseases. Thus, analyzing RNA-protein interactions are critical for understanding the mechanism of diseases and designing clinical strategies.
RNA-binding protein immunoprecipitation (RIP) has been used to interrogate RNA binding proteins (RBP) associated with specific RNA molecules. However, the experiments are not feasible because of the unavailability of antibodies for the particular RBP or low expression of the RBP. To overcome these issues epitope tags have been used for RIP experiments.
RNA-binding protein immunoprecipitation (RIP) has been used to interrogate RNA binding proteins (RBP) associated with specific RNA molecules. However, the experiments are not feasible because of the unavailability of antibodies for the particular RBP or low expression of the RBP. To overcome these issues epitope tags have been used for RIP experiments.
Features and Benefits
The GFP, FLAG-tagged SNRNP70 mRNA was developed to enable to perform control RIP experiments with RIPAb+ FLAG (Cat. No. 03-901) or RIPAb+ GFP (Cat. No. 03-902) RIP validated antibody and primer kits, and Magna RIP kit (Cat. # 17-700).
Storage and Stability
Store at -80ºC
Other Notes
The high amount of GFP-FLAG-tagged SNRNP70 protein is expressed immediately after transfection to cells and the well-known specific interaction of SNRNP70 and U1snRNA can be used for control RIP experiments with anti-GFP or anti-FLAG antibodies.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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