04-835-S
Anti-dimethyl-Histone H3 (Lys79) Antibody, clone NL59, Trial Size, rabbit monoclonal
culture supernatant, clone NL59, Upstate®
Synonym(s):
H3K79me2, Histone H3 (di methyl K79)
About This Item
Recommended Products
biological source
rabbit
Quality Level
antibody form
culture supernatant
antibody product type
primary antibodies
clone
NL59, monoclonal
species reactivity
vertebrates, human
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable (ChIP-seq)
dot blot: suitable
multiplexing: suitable
western blot: suitable
isotype
IgG
shipped in
wet ice
target post-translational modification
dimethylation (Lys79)
Gene Information
human ... H3F3B(3021)
Related Categories
General description
Specificity
Immunogen
Application
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using either 4 µL of Negative Control Supernatant, or 4 µL of Anti-dimethyl-Histone H3 (Lys79) and the Magna ChIP A Kit (Cat. # 17-610). Successful immunoprecipitation of dimethyl-Histone H3 (Lys79) associated DNA fragments was verified by qPCR using Control Primers
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Chromatin Immunoprecipitation: A representative lot of this antibody has been shown by an independent laboratory to work in ChIP.
ChIP-Seq Analysis:
A representative lot of this antibody was used by an independent laboratory for ChIP-Seq. See Egelhofer, T.A., et al. (2011). See Easwaran, H., et al. (2012).See Suzuki, H., et al. (2011).
Dot Blotting: Specificity of a representative lot confirmed by the ability of a 1:2500 dilution of the antibody to recognize peptides corresponding to regions of histone H3 with various modifications
Dot Blot Analysis: Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No 04-835-S, Anti-dimethyl Histone H3 (Lys79) Antibody, clone NL59 (1:500 dilution). Proteins were visualized using a Donkey anti-rabbit IgG conjugated to HRP and a chemiluminescence detection system..
Epigenetics & Nuclear Function
Histones
Quality
Target description
Physical form
Storage and Stability
Legal Information
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wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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