04-856
Anti-TBK1 Antibody, clone AOW9, rabbit monoclonal
culture supernatant, clone AOW9, from rabbit
Synonym(s):
NF-kB-activating kinase, NF-kappa-B-activating kinase, TANK-binding kinase 1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
culture supernatant
antibody product type
primary antibodies
clone
AOW9, monoclonal
species reactivity
mouse, canine, human, chicken
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... TBK1(29110)
mouse ... Tbkbp1(73174)
General description
TBK, also known as IκB kinase (IKK)-related kinase NAK, belong to the serine/threonine IKK kinase family and is thought to play a role in NF-kappa-B (NF-κB) activation. The NF-κB complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NF-κB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NF-κB activation in response to certain growth factors. For example, the protein can form a complex with the IKB protein TANK and TRAF2 and release the NF-κB inhibition caused by TANK.
Specificity
Predicted to cross-react with mouse, dog, and chicken based on sequence homology.
TBK1; Does not cross-react with IKKε, IKKα or IKKβ.
Immunogen
Epitope: a.a. 642-645
Peptide corresponding to the sequence C...EEVS... (amino acids 642-645) of human TANK-binding kinase 1 (TBK1) with an N-terminal cysteine added for conjugation purposes.
Application
Detect TBK1 using this Anti-TBK1 Antibody, clone AOW9 validated for use in WB & IP.
Immunoprecipitation:
1-4 μL of a previous lot immunoprecipitated TBK1 from 100 μg of HEK293 RIPA lysate.
1-4 μL of a previous lot immunoprecipitated TBK1 from 100 μg of HEK293 RIPA lysate.
Research Category
Signaling
Apoptosis & Cancer
Signaling
Apoptosis & Cancer
Research Sub Category
Kinases & Phosphatases
Immunological Signaling
Kinases & Phosphatases
Immunological Signaling
Quality
Evaluated by western blot on RIPA lysates from HEK293 cells.
Western Blot Analysis:
A 1:500 to 1:2,000 of this antibody detected TBK1 in RIPA lysates from HEK293 cells.
Western Blot Analysis:
A 1:500 to 1:2,000 of this antibody detected TBK1 in RIPA lysates from HEK293 cells.
Target description
85 kDa
Linkage
Replaces: 05-856
Physical form
Cultured supernatant containing 0.05% sodium azide.
Storage and Stability
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
RIPA lysates from HEK293 cells.
RIPA lysates from HEK293 cells.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Katherine A Fitzgerald et al.
Nature immunology, 4(5), 491-496 (2003-04-15)
The transcription factors interferon regulatory factor 3 (IRF3) and NF-kappaB are required for the expression of many genes involved in the innate immune response. Viral infection, or the binding of double-stranded RNA to Toll-like receptor 3, results in the coordinate
Elsa Salzer et al.
Journal of laboratory automation, 21(1), 133-142 (2015-07-15)
Chemoproteomics-based competition-binding assays allow the screening of compounds against endogenous proteins in cell or tissue extracts, but these assays are hampered by low throughput and high cost. Using compound pools rather than single compounds in a screening campaign holds the
Shihao Chen et al.
Frontiers in microbiology, 11, 603131-603131 (2020-12-29)
Avian leukosis virus subgroup J (ALV-J), an oncogenic retrovirus, is known to cause immunosuppression and various types of cancer in chickens. Recent reports have shown that epigenetic changes in DNA and chromatin are widely implicated in the life cycle of
Tao Deng et al.
Cancer research, 74(7), 2119-2130 (2014-02-04)
HER2(+) breast cancer is currently treated with chemotherapy plus anti-HER2 inhibitors. Many patients do not respond or relapse with aggressive metastatic disease. Therefore, there is an urgent need for new therapeutics that can target HER2(+) breast cancer and potentiate the
Sonia Sharma et al.
Science (New York, N.Y.), 300(5622), 1148-1151 (2003-04-19)
Rapid induction of type I interferon expression, a central event in establishing the innate antiviral response, requires cooperative activation of numerous transcription factors. Although signaling pathways that activate the transcription factors nuclear factor kappaB and ATF-2/c-Jun have been well characterized
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