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05-1403

Sigma-Aldrich

Anti-Giα2 Antibody, clone L5

clone L5, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

L5, monoclonal

species reactivity

guinea pig (43%), rat (69%), bovine (100%), human (62%), mouse (69%)

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG2b

NCBI accession no.

NM_002070.2

UniProt accession no.

P04899

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... GNAI2(2771)

Related Categories

Specificity

Specificity confirmed by Western blot versus bovine Gαt, rat Goα, rat Giα-1, Giα-2, rat Gia-3, bovine Gsα, and murine GoαA and GoαB.
This antibody recognizes Giα-2.

Immunogen

Recombinant Giα-2, purified by the method of Linder (J. Biol. Chem. [1990] 265, 8243).

Application

Optimal working dilutions must be determined by end user.

Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of G-Protein Monoclonal Antibody: Testicular Cancer

Western Blot: A previous lot of this antibody was used at 1:500 - 1:1000, with detection by 125l-labeled anti-IgG. Labels a single protein band of 39 - 42 kDa in bovine and rat membranes
Research Category
Signaling
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
This Anti-Giα2 Antibody, clone L5 is validated for use in WB, IH(P) for the detection of Giα2.

Quality

Routinely evaluated by Western Blot on Huvec lysates.

Western Blot Analysis: 1:500 dilution of this lot detected G I ALPHA 2 on 10 μg of Huvec lysates.

Target description

Labels a single protein band of 39-42 kDa in bovine and rat membranes.

Linkage

Replaces: MAB3077

Physical form

Format: Purified
Purified
Purified mouse monoclonal IgG2b, in 0.02 M phosphate buffer, 0.25 M sodium chloride, pH 7.6, with 0.1% sodium azide.

Storage and Stability

Maintain at 2-8°C for 1 year from date of receipt.

Analysis Note

Control
Huvec cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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