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05-1570

Sigma-Aldrich

Anti-PRDM1 (BLIMP-1) Antibody, clone 6D3

clone 6D3, from rat

Synonym(s):

PR domain containing 1, with ZNF domain, PR-domain zinc finger protein 1, beta-interferon gene positive-regulatory domain I binding factor, Positive regulatory domain I-binding factor 1, PRDI-binding factor-1, Beta-interferon gene positive regulatory dom

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6D3, monoclonal

species reactivity

mouse, human

technique(s)

ELISA: suitable
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_031574

UniProt accession no.

Q60636

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PRDM1(639)

Related Categories

General description

B-lymphocyte induced maturation protein (BLIMP-1) is a 100 kDa transcriptional repressor containing 1 DET domain and 4 C2H2-type zinc fingers. In the nucleus, BLIMP-1 is known to bind specifically with the Beta-interferon gene positive regulatory domain I (PRDI), and to interact with PRMT5. During B-cell differentiation, BLIMP-1 actively represses the transcription of the gene, c-myc, through binding its promoter and has been deemed a “master regulator” for terminal B-cell differentiation, as it is able to initiate the BCL1 cell developmental cascade.

Specificity

Recognizes BLIMP-1

Immunogen

Epitope: Recognizes epitope located between amino acids 255-395 of BLIMP-1
Recombinant protein corresponding to mouse BLIMP-1.

Application

ELISA Analysis: A representative lot was used by an independent laboratory in ELISA. (Kallies, A., et al. (2004). J Exp Med. 200(8):967-977.)

Flow Cytometry Analysis: A representative lot was used by an independent laboratory in FC. (Kallies, A., et al. (2004). J Exp Med. 200(8):967-977.)
Research Category
Epigenetics & Nuclear Function

Inflammation & Immunology
Use Anti-PRDM1 (BLIMP-1) Antibody, clone 6D3 (Rat Monoclonal Antibody) validated in WB, ELISA, IHC, FC to detect PRDM1 (BLIMP-1) also known as PR-domain zinc finger protein 1.

Quality

Evaluated by Western Blot in HL-60 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected BLIMP-1 on 10 µg of HL-60 cell lysate.

Target description

~ 95 kDa observed

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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