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Sigma-Aldrich

Anti-GRB2 Antibody, clone 3F2

clone 3F2, Upstate®, from mouse

Synonym(s):

Anti-ASH, Anti-EGFRBP-GRB2, Anti-Grb3-3, Anti-MST084, Anti-MSTP084, Anti-NCKAP2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

3F2, monoclonal

species reactivity

bovine, mouse, hamster, rat, human

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NM_002086

UniProt accession no.

P62993

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... GRB2(2885)

Specificity

N-terminal SH3 region of GRB2

Immunogen

Recombinant GRB2/Ash

Application

This Anti-GRB2 Antibody, clone 3F2 is validated for use in IH, WB for the detection of GRB2.

Quality

routinely evaluated in immunoblot on RIPA lysates of non-stimulated human A431 carcinoma cells, RIPA lysates of mouse 3T3/A31 fibroblasts, rat L6 myoblasts, Chinese hamster ovary cells or bovine brain extract

Target description

23-25kDa

Physical form

Format: Purified
PBS containing 50% glycerol

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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c-Cbl is transiently tyrosine-phosphorylated, ubiquitinated, and membrane-targeted following CSF-1 stimulation of macrophages
Wang, Y., et al
The Journal of Biological Chemistry, 271, 17-20 (1996)
A novel protein kinase C alpha-dependent signal to ERK1/2 activated by alphaVbeta3 integrin in osteoclasts and in Chinese hamster ovary (CHO) cells.
Rucci, N; DiGiacinto, C; Orru, L; Millimaggi, D; Baron, R; Teti, A
Journal of Cell Science null
Protein-tyrosine phosphatase 1D modulates its own state of tyrosine phosphorylation
Stein-Gerlach, M., et al
The Journal of Biological Chemistry, 270, 24635-24637 (1995)
M Rozakis-Adcock et al.
Nature, 363(6424), 83-85 (1993-05-06)
Many tyrosine kinases, including the receptors for hormones such as epidermal growth factor (EGF), nerve growth factor and insulin, transmit intracellular signals through Ras proteins. Ligand binding to such receptors stimulates Ras guanine-nucleotide-exchange activity and increases the level of GTP-bound
Y Chen et al.
The Journal of biological chemistry, 273(31), 19809-19816 (1998-07-25)
While it is well established that adenylyl cyclase and phospholipase C-beta are two proximal signal effectors for the calcitonin receptor, the more distal signaling pathways are less well characterized. G protein-coupled receptors can activate Erk1/2 by Gs-, Gi-, or Gq-dependent
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