05-644
Anti-CAR Antibody, clone RmcB
clone RmcB, Upstate®, from mouse
Synonym(s):
46 kD coxsackievirus and adenovirus receptor (CAR) protein, CVB3 binding protein, CVB3-binding protein, Coxsackievirus B-adenovirus receptor, coxsackie virus B receptor, coxsackie virus and adenovirus receptor
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All Photos(4)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
RmcB, monoclonal
species reactivity
human, rat, mouse
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CXADR(1525)
General description
The coxsackievirus and adenovirus receptor (CAR) mediates cell attachment and infection by coxsackie B viruses and by a number of adenoviruses. CAR also mediates homotypic intercellular interactions. In polarized epithelial cells, CAR is closely associated with the tight junction, where it contributes to the barrier to paracellular flow of solutes and macromolecules. CAR′s biological roles are not well defined, but emerging evidence suggests that it may function during embryonic development and in regulating cell proliferation.
Specificity
Recognizes CAR at MW ~46 kDa.
Immunogen
Native human coxsackie virus-adenovirus receptor protein.
Application
Anti-CAR Antibody, clone RmcB detects level of CAR & has been published & validated for use in IC & WB.
Immunocytochemistry:
1-2 μg/mL of a previous lot showed positive immunostaining for CAR in HeLa cells, hCAR transfected CHO cells, but not in untransfected CHO cells fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton-X.
1-2 μg/mL of a previous lot showed positive immunostaining for CAR in HeLa cells, hCAR transfected CHO cells, but not in untransfected CHO cells fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton-X.
Quality
Evaluated by Western Blot on mouse small intestine lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected Car on 10 µg of mouse small intestine lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected Car on 10 µg of mouse small intestine lysates.
Target description
~46 kDa
Physical form
Format: Purified
Purified mouse monoclonal IgG1 in buffer containing 0.1M Tris-Glycine, 0.15M NaCl and 0.05% Sodium Azide, pH 7.4.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Certificates of Analysis (COA)
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Klaus Heger et al.
European journal of immunology, 45(6), 1614-1620 (2015-03-20)
Replication-deficient recombinant adenoviruses are potent vectors for the efficient transient expression of exogenous genes in resting immune cells. However, most leukocytes are refractory to efficient adenoviral transduction as they lack expression of the coxsackie/adenovirus receptor (CAR). To circumvent this obstacle
Kenneth J Mandell et al.
Investigative ophthalmology & visual science, 48(9), 3928-3936 (2007-08-29)
Junctional adhesion molecules (JAMs) are a family of adhesion proteins found in intercellular junctions. Evidence suggests that JAM-A is important for the regulation of tight junction assembly and epithelial barrier function. The authors recently reported that JAM-A is expressed in
Anton V Borovjagin et al.
PloS one, 6(10), e24281-e24281 (2011-10-18)
To explore gene therapy strategies for amelogenesis imperfecta (AI), a human ameloblast-like cell population was established from third molars of an AI-affected patient. These cells were characterized by expression of cytokeratin 14, major enamel proteins and alkaline phosphatase staining. Suboptimal
William C Adams et al.
The Journal of general virology, 90(Pt 7), 1600-1610 (2009-03-14)
The coxsackievirus-adenovirus receptor (CAR) is the described primary receptor for adenovirus serotype 5 (Ad5), a common human pathogen that has been exploited as a viral vector for gene therapy and vaccination. This study showed that monocytes and dendritic cells (DCs)
Antitumor effects of bladder cancer-specific adenovirus carrying E1A-androgen receptor in bladder cancer.
Zhai, Z; Wang, Z; Fu, S; Lu, J; Wang, F; Li, R; Zhang, H; Li, S; Hou, Z; Wang, H; Rodriguez, R
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