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06-1041

Sigma-Aldrich

Anti-RIG-I, CT Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Probable ATP-dependent RNA helicase DDX58, DEAD box protein 58, Retinoic acid-inducible gene 1 protein, RIG-1, Retinoic acid-inducible gene I protein, RIG-I

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

western blot: suitable

NCBI accession no.

NP_055129

UniProt accession no.

O95786

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... DDX58(23586)

General description

Retinoic acid-inducible gene 1 (RIG-1, DDX58) is a cytoplasmic protein belonging to the family of RIG-1-like receptor (RLR) family. It is a multidomain protein containing an N-terminal caspase recruitment domain (CARD); a DExD/H box helicase domain; and a C-terminal repression domain. RIG-1 has a well-documented role in innate immunity, as a virus-sensing protein that detects viral RNA molecules and induces the production of IFN-β through activation of the inflammasome complex.

Specificity

This antibody recognizes the C-terminus of RIG-I.

Immunogen

Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human RIG-I.

Application

Anti-RIG-I, C-terminus Antibody is an antibody against RIG-I, C-terminus for use in Western Blotting.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology

Quality

Evaluated by Western Blot in untreated A549 cell lysate and A549 cell lysate infected with 40 HAU/mL of SeV for 12 hours.

Western Blot Analysis: 1 µg/mL of this antibody detected RIG-I in 10 µg of untreated A549 cell lysate and A549 cell lysate infected with 40 HAU/mL of SeV for12 hours..

Target description

~102 kDa observed

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Untreated A549 cell lysate and A549 cell lysate infected with 40 HAU/mL of SeV for12 hours.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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