Anti-NFκB p52 Antibody

The NFκB transcription factor was originally identified as a protein complex consisting of a 65 kDa DNA binding subunit and an associated 50 kDa protein. The 65 kDa subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino terminus of a precursor designated p105. A cDNA has been isolated that encodes an alternative DNA binding subunit of NFκB. It is synthesized as a 100 kDa protein that is expressed in a variety of cell types and, like p105, undergoes cleavage to generate its NFκB subunit, in this case a protein designated p52 (previously referred to as p49). In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells.

Recognizes NFκB p52 and its precursor.

KLH conjugated synthetic peptide (CYNPGLDGIIEYDDFK) contained within amino acids 4-19 of human NF κB, p52

Detect NFκB p52 with Anti-NFκB p52 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in IP & WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

routinely evaluated by immunoblot on RIPA lysates from Raji cells

52 kDa, and precursor (100 kDa)

Format: Purified

Protein A Purified immunoglobulin in in 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% NaN3 as a preservative.

Unpurified

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Control
Raji cell lysate, Jurkat whole cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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