Anti-PP2A Antibody, B β subunit

PP2A is a heterotrimeric type 2 Ser/Thr phosphatase, composed of a structural subunit (A), a regulatory subunit (B), and a catalytic subunit (C). The structural A and catalytic C subunits form the constitutive core of the enzyme, which associates with one of a large number of regulatory B subunits. There are multiple isotypes (encoded by distinct genes) of the B subunit, grouped into three families: B/PR55, B′/PR61, and B′′/PR72 . The prototypical B (or PR55) family of subunits is encoded by three genes, Balpha, Bbeta and Bgamma . The B′ (or B56) family consists of at least seven splice variants derived from five genes. RNA′s for the two members of the B′′ family, PR72 and PR130, arise by alternative splicing or use of alternate transcription start sites from the same gene. Primary structures as well as functions of B and B′ subunits are conserved from yeast to mammals. PTPA is a member of the B′ family that is required for activation of PP2A by an undefined mechanism. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and its high sensitivity to Okadaic Acid. It is also thought to be the principal downmodulator of Ser/Thr kinase cascades.

Recognizes Protein Phosphatase 2A/B. Minimal cross reactivity against 2A/B.

The immunogen used to the purified peptide conjugated to KLH corresponding to the sequence NH2-Cys-Gly-Glu-Glu-Asp-Ile-Asp-THr-Arg- Lys-Ile-Asn-Asn-Ser-Phe-COOH.

Anti-PP2A Antibody, B β subunit detects level of PP2A & has been published & validated for use in WB, IH(P).

Research Category
Signaling

Research Sub Category
PI3K, Akt, & mTOR Signaling

Kinases & Phosphatases

Routinely evaluated by Western Blot on NIH/3T3 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected PP2A/B BETA on 10 μg of NIH/3T3 lysates.

~55 kDa

Replaces: AB4086

Affinity purified

Purified rabbit polyclonal IgG provided as 0.2 µm sterile filtered solution in phosphate buffered saline with 0.08% sodium azide.

Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
NIH/3T3 Cell Lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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