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07-1512

Sigma-Aldrich

Anti-phospho-WAVE2 (Ser343) Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

SCAR2, WASF2, dJ393P12.2, WASP family protein member 2, Protein WAVE-2, Verprolin homology domain-containing protein 2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, monkey

technique(s)

western blot: suitable

NCBI accession no.

NP_008921

UniProt accession no.

Q9Y6W5

shipped in

wet ice

target post-translational modification

phosphorylation (pSer343)

Gene Information

human ... WASF2(10163)

General description

Wiskott-Aldrich Syndrome Protein (WASP)-family Protein Member 2 (known as WAVE2) is found in many cells and tissues, with strong expression in peripheral blood leukocytes. WAVE2 is involved in signaling RTKs and small GTPases. As a whole, the WAVE family of proteins have a general function for regulating the actin cytoskeleton in various tissues.

Specificity

This antibody recognizes WAVE2 phosphorylated at Ser343.

Immunogen

Epitope: Phosphorylated Ser343
KLH-conjugated linear peptide corresponding to human WAVE2 phosphorylated at Ser343.

Application

Anti-phospho-WAVE2 (Ser343) Antibody detects level of phospho-WAVE2 (Ser343) & has been published & validated for use in WB.
Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling
Western Blot Analysis: A previous lot of this antibody was used to detect endogenous phospho-WAVE2 (Ser343) in COS-7 and HMECs following EGF stimulation Ā± pretreatment with U0126 (Mendoza, M.C., et al. (2011). Mol Cell. 41(6):661-671).

Quality

Evaluated by Western Blot in COS-7+EGF cell lysates untreated and lambda phosphatase-treated.

Western Blot Analysis: A 1:500 dilution of this antibody detected phospho-WAVE2 (Ser343) in 10 Āµg of COS-7+EGF cell lysates untreated and lambda phosphatase treated.

Target description

~75 kDa observed.
An uncharacterized band appears at ~103 kDa in some lysates.

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8Ā°C from date of receipt.

Analysis Note

Control
COS-7+EGF cell lysates untreated and lambda phosphatase treated.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a productā€™s label following the words ā€˜Lotā€™ or ā€˜Batchā€™.

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ERK-MAPK drives lamellipodia protrusion by activating the WAVE2 regulatory complex.
Mendoza, MC; Er, EE; Zhang, W; Ballif, BA; Elliott, HL; Danuser, G; Blenis, J
Molecular Cell null
Andrea Palamidessi et al.
Nature materials, 18(11), 1252-1263 (2019-07-25)
During wound repair, branching morphogenesis and carcinoma dissemination, cellular rearrangements are fostered by a solid-to-liquid transition, known as unjamming. The biomolecular machinery behind unjamming and its pathophysiological relevance remain, however, unclear. Here, we study unjamming in a variety of normal

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