Anti-HuR Antibody

HuR (Hu-antigen R, ELAV-like protein 1, ELAV1) is an RNA bindnig protein that contains 3 RNA-binding domains. It is known to binds AU-rich elements if mRNA. It regulates gene expression by destabilizing their mRNA. It is known to be upregulated in certain cancers such as breast and gastric cancers. Some of its targets include cFos, IL-3, and BRCA1 mRNAs. HuR has also been shown to be involved in the regulation of VEFG-A and NFkB expression in certain cell types. It activation and accumulation are also linked to many energy signaling molcules such as that of the PI3K/Akt pathway and AMPK.

Detects HuR

Other species have not been tested.

Epitope: N-terminus

Synthetic peptide corresponding to the N-terminus of human HuR.

Immunofluorescent ICC Analysis:

Research Category
Signaling

Research Sub Category
Insulin/Energy Signaling

RNA Binding Protein (RBP)

Use Anti-HuR Antibody (Rabbit Polyclonal Antibody) validated in WB, IF to detect HuR also known as ELAV-like 1, Hu antigen R.

Western Blot Analysis:
The antibody detected HuR at 1:500-1:1,000 dilutions in Jurkat cell lysate resolved via SDS-PAGE and transferred to PVDF.

36 kDa

Replaces: AB5831

Affinity purified polyclonal precipitated in ammonium sulfate.

Rabbit polyclonal precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Control
Jurkat cell lysate

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