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07-1782

Sigma-Aldrich

Anti-RAD51 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

BRCA1/BRCA2-containing complex, subunit 5, DNA repair protein RAD51 homolog 1, RAD51 (S. cerevisiae) homolog (E coli RecA homolog), RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae), RAD51 homolog protein, RAD51, S.cerevisiae, homolog of, A, RecA-lik

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse

species reactivity (predicted by homology)

human, rat

technique(s)

western blot: suitable

NCBI accession no.

NP_002866

UniProt accession no.

Q06609

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... RAD51(5888)
mouse ... Deptor(97998)

General description

RAD51 (previously known as DNA repair protein RAD51 homolog 1) is a member of the Rec A family involved in DNA repair. RAD51 interacts with BRCA1 and BRCA2, which are both involved with breast cancer, to activate homologous recombination and DNA damage repair. BRCA1 appears to be present in heredity forms of breast cancer that results in the transition of G to A on codon 150, thereby converting ARG to GLN. BRCA2 regulates intercellular localization and DNA binding ability. Loss of BRCA2 results in genomic instability and tumorgenesis. RAD51 acts as ATPase to repair double strand breaks and also unwinds DNA to form a helical nucleoprotein filament. RAD51 is predominantly expressed in the testis and thymus but can also be found in the small intestine, placenta, colon, pancreas and ovaries.

Specificity

This antibody recognizes RAD51.

Immunogen

Synthetic peptide corresponding to RAD51.

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Use Anti-RAD51 Antibody (rabbit polyclonal antibody) validated in WB to detect RAD51 also known as BRCA1/BRCA2-containing complex subunit 5 ,DNA repair protein RAD51 homolog 1, RecA-like protein ,recombination protein A.

Quality

Western Blot Analysis:
1:500 dilution of this antibody was used to detect RAD51 on mouse testis lysate.

Target description

37 kDa

Linkage

Replaces: MABE515

Physical form

Affinity purified
Purified rabbit polyclonal antibody precipitated in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

Storage and Stability

Stable for 1 year at 2-8ºC from date of receipt.To reconstitute the antibody, centrifuge the antibody at a moderate speed (5,000 rpm) for 5 minutes. Carefully remove the ammonium sulfate/PBS buffer solution and discard; 10μL of residual ammonium sulfate solution will not affect the re-suspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Re-suspend the antibody pellet in 100L either standard PBS or TBS (pH 7.3-7.5). DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to re-suspend are normal. Vials are overfilled to compensate for any losses.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Sameer Mirza et al.
Cell cycle (Georgetown, Tex.), 11(22), 4266-4274 (2012-10-26)
Cell cycle regulation and DNA repair following damage are essential for maintaining genome integrity. DNA damage activates checkpoints in order to repair damaged DNA prior to exit to the next phase of cell cycle. Recently, we have shown the role

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