Anti-phospho eNOS Antibody (Ser1177)

NO, produced by nitric oxide synthases (NOS), is a critical signaling molecule involved in many physiological processes including muscle contraction, vasodilation, neuronal transmission, and immune responses. Some of these effects are mediated through reaction with and activation of guanylate cyclase (GC), resulting in increased cGMP production. With a half-life of seconds, NO readily diffuses through the cytosol and cell membrane, allowing it to act inside or adjacent to the cell in which it was produced. Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).

Epitope: Near C-terminus

KLH-conjugated linear peptide corresponding to a region near the C-terminus of human eNOS phosphorylated at Ser1177.

Anti-phospho eNOS Antibody (Ser1177) is a highly specific rabbit polyclonal antibody, that targets eNOS/NOS III & has been tested in western blotting.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Evaluated by Western Blotting in untreated and VEGF treated HUVEC cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected phospho eNOS (Ser1177) in 10 µg of VEGF treated HUVEC cell lysate.

~133 kDa observed

Replaces: 07-428

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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