07-582-I
Anti-phospho-IRF-3 (Ser396) Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Interferon regulatory factor 3, IRF-3
Sign Into View Organizational & Contract Pricing
All Photos(3)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
species reactivity (predicted by homology)
porcine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer396)
Gene Information
human ... IRF3(3661)
pig ... Irf3(396656)
rhesus monkey ... Irf3(718970)
General description
Interferon regulatory factors (IRFs) comprise a family of transcription factors that function with the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection. IRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response. In unstimulated cells, IRF-3 is present in the cytoplasm. Viral infection results in phosphorylation of IRF-3 and leads to its translocation to the nucleus where it activates promoters containing IRF-3-binding sites. Phosphorylation of IRF-3 occurs at a cluster of C-terminal serine and threonine residues (between 385 and 405) leading to its association with the p300/CBP coactivator protein that promotes DNA binding and transcriptional activity.
Specificity
This antibody recognizes IRF-3 phosphorylated at Ser396.
Immunogen
Epitope: SNS → ANA: Mutation of this residue results in loss of viral-induced phosphorylation of IRF-3.
KLH-conjugated linear peptide corresponding to human IRF-3 phosphorylated at Ser396.
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Use Anti-phospho-IRF-3 (Ser396) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect phospho-IRF-3 (Ser396) also known as Interferon regulatory factor 3.
Quality
Evaluated by Western Blot in untreated and sendai virus treated A549 cell lysates.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected phospho-IRF-3 (Ser396) in 10 µg of sendai virus treated A549 cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected phospho-IRF-3 (Ser396) in 10 µg of sendai virus treated A549 cell lysate.
Target description
~50 kDa observed. Uncharacterized bands may appear at ~40 kDa and ~80 kDa in some lysates.
Linkage
Replaces: 07-582
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Untreated and sendai virus treated A549 cell lysates
Untreated and sendai virus treated A549 cell lysates
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
recommended
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Cortisol is a potent modulator of lipopolysaccharide-induced interferon signaling in macrophages.
Anja M Billing,Fred Fack,Jonathan D Turner,Claude P Muller
Innate Immunity null
Mitsuaki Mori et al.
The Journal of biological chemistry, 279(11), 9698-9702 (2004-01-03)
Interferon regulatory factor (IRF)-3 is a critical transcription factor regulating innate immune responses against viral and bacterial infections. Signals activated by various pathogens are integrated by IRF-3 kinase, resulting in the specific phosphorylation of IRF-3 in the cytoplasm. This phosphorylation
Jim Boonyaratanakornkit et al.
Journal of virology, 85(4), 1495-1506 (2010-12-03)
Human parainfluenza virus type 1 (HPIV1) is an important respiratory pathogen in young children, the immunocompromised, and the elderly. We found that infection with wild-type (WT) HPIV1 suppressed the innate immune response in human airway epithelial cells by preventing not
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service