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Sigma-Aldrich

Orange G (C.I. 16230)

for microscopy Certistain®

Synonym(s):

Orange G (C.I. 16230)

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About This Item

Empirical Formula (Hill Notation):
C16H10N2Na2O7S2
CAS Number:
1936-15-8
Molecular Weight:
452.37
MDL number:
MFCD00012457
UNSPSC Code:
41116130
EC Index Number:
217-705-6

Quality Level

400

form

solid

potency

>3000 mg/kg LD50, oral (Rat)

composition

Dye, ≥80% spectrophotometric assay

pH

9 (20 °C, 10 g/L in H2O)

solubility

70 g/L

bulk density

430 kg/m3

εmax

380-500 at 476-481 nm in water at 0.015 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

InChI

1S/C16H12N2O7S2.2Na/c19-13-7-6-10-8-12(26(20,21)22)9-14(27(23,24)25)15(10)16(13)18-17-11-4-2-1-3-5-11;;/h1-9,17H,(H,20,21,22)(H,23,24,25);;/q;2*+1/p-2/b18-16+;;

InChI key

AEOVEGJBKQQFOP-DDVLFWKVSA-L

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General description

The dry dye Orange G (C.I. 16230) for microscopy Certistain®, is used for the preparation of a staining solution for Papanicolaou′s staining in cytology in material of human origin. The effect of the Orange G staining solution is considerable in smears with keratinized cells under acidic pH conditions, when the obvious orangeophilic cytoplasmic stain is recognizable. As a Certistain® dye it is chemically quality-certified according to highest standards and strict specifications. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (spectrophotometrically): ≥ 80 %
Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (water): 476 - 481 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.015 g/l; water): 380 - 500
TLC-Test: passes test
Loss on drying (110 °C): ≤ 15 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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